CRISPR/Cas9 Customization of Electronic Genome Mapping for Targeted Analysis

The standard electronic genome mapping (EGM) workflow covers more than 90% of the human genome, but regions with insufficient or excessive nick density can limit contig alignment and structural variant detection. This poster demonstrates how CRISPR/Cas9 can be used to selectively add or block nick sites within the OhmX™ Genome Preparation workflow—unlocking previously inaccessible regions with minimal protocol changes.

Targeted blocking near AKT1 restored contig alignment in a region previously inaccessible with standard EGM chemistry, while a combined add-and-block strategy optimized interval sizing at the FMR1 locus for Fragile X repeat expansion quantification using the RepX™ Repeat Expansion Analysis pipeline. The poster also presents proof-of-concept data showing that EGM can characterize sgRNA efficiency and Cas9 enzyme performance at the base-pair level.

Explore how CRISPR-enhanced EGM empowers more targeted structural variant research—and extends the reach of the OhmX Platform across the genomic regions that matter most to your work.